import pandas as pd

df = pd.DataFrame([[1, 2, 3, 4, 2, 3, 4, 2, 3, 4], [5, 6, 7, 8, 2, 3, 4, 2, 3, 4]], index=['A', 'B'], columns=['One', 'Two', 'Three', 'Four', '', '', '', '', '', ''])
print(df)
df.iloc[0][1] = 55
print(df)